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Progen Biotechnik nhek cell lines
Nhek Cell Lines, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Structure-guided identification and biological evaluation of reversible EGFR C797S inhibitors.
Article Snippet: HEK293 and NHEK cell lines were provided and maintained by PROGEN Biyoteknoloji Ltd. Şti. (Istanbul, Turkey) under standard culture conditions.



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Fig. 22. Short-term cytotoxicity after 1 h (A) and 3 h (B) incubation with tested materials on <t>NHEK</t> cells.
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Fig. 22. Short-term cytotoxicity after 1 h (A) and 3 h (B) incubation with tested materials on <t>NHEK</t> cells.
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The level of IL-4 ( A and B ) and IL-13 ( C and D ) after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial <t>Keratinocytes</t> <t>(NHEK;</t> ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001) are marked.
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The level of IL-4 ( A and B ) and IL-13 ( C and D ) after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial <t>Keratinocytes</t> <t>(NHEK;</t> ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001) are marked.
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The level of IL-4 ( A and B ) and IL-13 ( C and D ) after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial <t>Keratinocytes</t> <t>(NHEK;</t> ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001) are marked.
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The level of IL-4 ( A and B ) and IL-13 ( C and D ) after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial <t>Keratinocytes</t> <t>(NHEK;</t> ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001) are marked.
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The level of IL-4 ( A and B ) and IL-13 ( C and D ) after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial <t>Keratinocytes</t> <t>(NHEK;</t> ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001) are marked.
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Fig. 22. Short-term cytotoxicity after 1 h (A) and 3 h (B) incubation with tested materials on NHEK cells.

Journal: International journal of biological macromolecules

Article Title: Design and development of multibiocomponent hybrid alginate hydrogels and lipid nanodispersion as new materials for medical and cosmetic applications.

doi: 10.1016/j.ijbiomac.2024.134405

Figure Lengend Snippet: Fig. 22. Short-term cytotoxicity after 1 h (A) and 3 h (B) incubation with tested materials on NHEK cells.

Article Snippet: Normal human dermal fibroblast (NHDF) and normal human keratinocytes (NHEK) cell lines were purchased from PromoCell.

Techniques: Incubation

Fig. 23. Long-term cytotoxicity on NHDF (A) and NHEK (B) cells. Cells were pre-incubated with tested materials for 1 h and 3 h.

Journal: International journal of biological macromolecules

Article Title: Design and development of multibiocomponent hybrid alginate hydrogels and lipid nanodispersion as new materials for medical and cosmetic applications.

doi: 10.1016/j.ijbiomac.2024.134405

Figure Lengend Snippet: Fig. 23. Long-term cytotoxicity on NHDF (A) and NHEK (B) cells. Cells were pre-incubated with tested materials for 1 h and 3 h.

Article Snippet: Normal human dermal fibroblast (NHDF) and normal human keratinocytes (NHEK) cell lines were purchased from PromoCell.

Techniques: Incubation

The level of IL-4 ( A and B ) and IL-13 ( C and D ) after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial Keratinocytes (NHEK; ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001) are marked.

Journal: Journal of Inflammation Research

Article Title: The Effect of Osthole on Transient Receptor Potential Channels: A Possible Alternative Therapy for Atopic Dermatitis

doi: 10.2147/JIR.S425978

Figure Lengend Snippet: The level of IL-4 ( A and B ) and IL-13 ( C and D ) after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial Keratinocytes (NHEK; ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001) are marked.

Article Snippet: In brief, the Normal Human Epidermal Keratinocyte (NHEK) cell line was purchased from PromoCell GmbH (Heidelberg, Germany, cat. no. C-12005) and cultured in T-75 flasks in keratinocyte medium (Keratinocyte Growth Medium 2 ready to use, PromoCell, Heidelberg, Germany, cat. No. C-20011).

Techniques: Incubation, Standard Deviation, Control

Histamine receptor 4 ( A and B ) and IL-4 receptor-α ( C and D ) gene expression level after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial Keratinocytes (NHEK; ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS (# p < 0.05, ### p < 0.001, #### p < 0.0001) are marked.

Journal: Journal of Inflammation Research

Article Title: The Effect of Osthole on Transient Receptor Potential Channels: A Possible Alternative Therapy for Atopic Dermatitis

doi: 10.2147/JIR.S425978

Figure Lengend Snippet: Histamine receptor 4 ( A and B ) and IL-4 receptor-α ( C and D ) gene expression level after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial Keratinocytes (NHEK; ( A and C )) and prepared model of 3D skin (3D skin; ( B and D )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS (# p < 0.05, ### p < 0.001, #### p < 0.0001) are marked.

Article Snippet: In brief, the Normal Human Epidermal Keratinocyte (NHEK) cell line was purchased from PromoCell GmbH (Heidelberg, Germany, cat. no. C-12005) and cultured in T-75 flasks in keratinocyte medium (Keratinocyte Growth Medium 2 ready to use, PromoCell, Heidelberg, Germany, cat. No. C-20011).

Techniques: Gene Expression, Incubation, Standard Deviation, Control

TRPV1 ( A and B ), TRPV4 ( C and D ) and TRPM8 ( E and F ) gene expression level after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial Keratinocytes (NHEK; ( A, C and E )) and prepared model of 3D skin (3D skin; ( B, D and F )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ### p < 0.001, #### p < 0.0001) are marked.

Journal: Journal of Inflammation Research

Article Title: The Effect of Osthole on Transient Receptor Potential Channels: A Possible Alternative Therapy for Atopic Dermatitis

doi: 10.2147/JIR.S425978

Figure Lengend Snippet: TRPV1 ( A and B ), TRPV4 ( C and D ) and TRPM8 ( E and F ) gene expression level after incubation with histamine (Hist; 100 µg/mL), lipopolysaccharides (LPS; 2 µg/mL) alone and in mixtures with osthole (OST; 0.125 and 0.5 mg/mL) and clobetasol propionate (CP; 0.5 mg/mL) in Normal Human Epithelial Keratinocytes (NHEK; ( A, C and E )) and prepared model of 3D skin (3D skin; ( B, D and F )). The horizontal line shows the mean and the bars show the standard deviation. Statistically significant differences (Two-way ANOVA with Tukey’s multiple comparisons test) compared to control (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and to cells treated with histamine or LPS ( # p < 0.05, ### p < 0.001, #### p < 0.0001) are marked.

Article Snippet: In brief, the Normal Human Epidermal Keratinocyte (NHEK) cell line was purchased from PromoCell GmbH (Heidelberg, Germany, cat. no. C-12005) and cultured in T-75 flasks in keratinocyte medium (Keratinocyte Growth Medium 2 ready to use, PromoCell, Heidelberg, Germany, cat. No. C-20011).

Techniques: Gene Expression, Incubation, Standard Deviation, Control